Labor / Methoden

Molecular cloning procedures:

- polymerase chain reaction (PCR)

- DNA gel electrophoresis

- variety of cloning and expresson vectors

Extensive expertise in (membrane) protein expression (incl. bio-reactors)

Subcellular fractionation

Detergent & polymer screening for membrane protein isolation

Chromatography-based protein purification

Protein modification (site-specific labeling, biotinylation etc)

Protein reconstitution into model membranes (liposomes, nanodiscs, supported lipid membranes, GUVs)

Fluorescence-based analysis of macromolecular complexes

Surface plasmon resonance for studying protein:protein and protein:ligand interactions

Quartz crystal microbalance with dissipation (e.g. protein:lipid interactions)

Single-particle tracking in lipid membranes (coopeartion with Prof. Dr. Cornelia Monzel)

Structural analysis:

- cryo-electron microscopy (cooperation with Prof. Dr. Roland Beckmann, LMU Munich)

- crystallography & small-angle X-ray scattering (Center of Structural Studies)